Population genetic analysis and molecular marker analysis (Five day short course for advanced technicians)
Pre-requisite: DNA fingerprinting of cocoa (Theory and practice)
Course description: Introduction to population genetics. Methods of studying genetic diversity. Hardy-Weinberg equilibrium and its applications. The use of genetic software to analyse DNA fingerprinting data – determine genetic diversity, develop phylogenetic trees to illustrate relationships between populations. Use of genetic information to determine core collections. Use of STRUCTURE to determine phylogeny and relationships. Principles of molecular markers and methodologies to determine molecular markers. Using software to carryout genome-wide association studies and determine phenotype-marker associations. Validating molecular markers using forward and reverse genetics. Establishment of biparental populations and QTL analysis. At the end of the course, students will be able to understand the theoretical and practical methods of using DNA fingerprint data in population genetic study, germplasm conservation and developing and using molecular markers.
Resources: Laboratory consumables, printing services and office support. Provision of breaks, bench fee.